Encystation of Acanthamoeba castellanii : dye uptake for assessment by flow cytometry and confocal laser scanning microscopy

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Encystation of Acanthamoeba castellanii : dye uptake for assessment by flow cytometry and confocal laser scanning microscopy

Article

CONNELL (C.) | RUTTER (A.) | HILL (B.) | SULLER (M.) | LLOYD (D.)

Source : - 2001

Aims: To develop rapid means of distinguishing between cysts and trophozoites of the opportunistic pathogen, Acanthamoeba castellanii, the causative agent of keratitis. Methods and Results: Fluorescence of Congo Red, Calcoflor White was specific for the endocyst wall; trophozoites did not become fluorescent. The anionic oxonol dye, DiBAC4(3), did not penetrate the cytoplasmic membrane after short-term (<5 min) exposure, whereas cysts are permeable and become fluorescent. Confocal scanning laser microscopy confirmed these properties and large populations of organisms were analysed by flow cytometry. Conclusions: These data provide a rapid alternative to traditional haemocytometer or plate counts for discrimination of trophozoites from cysts. Significance and Impact of the Study: Rapid and precise determination of the growth cycle of a dangerous ocular pathogen (Résumé en anglais ; cop. The Society for applied microbiology)

See the journal "Journal of Applied Microbiology"

Other issues of the journal "Journal of Applied Microbiology"

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Extr. de : Extr. de : Journal of applied microbiology, ISSN 1364-5072, vol. 90, n° 5, 2001, p. 706-712. Résumé en anglais. Bibliogr.

Physical description: 7 p. : ill. en noir et blanc ; 30 cm.

Publication date: 2001

Journal: Journal of Applied Microbiology

ISSN: 1364-5072

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